Application

Research CategoryInflammation & Immunology

Biochem/physiol Actions

Target Sub-Family: Lipid/Atypical

Protein Target: mTOR (FRAP1)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

N-terminal FLAG-tagged, recombinant, human mTOR, amino acids 1362-end

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Packaging

Also available in 100µg size, please inquire for pricing and availability.

Physical form

16µg of enzyme in 100µl of 10mM HEPES, 50mM NaCl, 50mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 300µg/ml FLAGTM peptide, 20% v/v glycerol

Storage and Stability

Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Unit Definition

Specific Activity: where one unit of mTOR activity is defined as 1 nmole of phosphate incorporated into 2mg/ml mTOR substrate per minute at 30°C with a final ATP concentration of 100µM.